Sticky ends from different SexAI sites may not be compatible.Ĭleavage may be enhanced when more than one copy of the XmaI recognition sequence is present. Sticky ends from different CsiI sites may not be compatible.
Sticky ends from different DraIII sites may not be compatible. Sticky ends from different BbsI sites may not be compatible.ībsI gradually loses activity when stored at -20☌. Sticky ends from different EcoO109I sites may not be compatible.Įfficient cleavage requires at least two copies of the SacII recognition sequence. PaeR7I does not recognize the sequence CTCTCGAG. Prolonged incubation with NdeI may lead to removal of additional nucleotides.Īfter cleavage, BamHI-HF® (but not the original BamHI) can remain bound to DNA and alter its electrophoretic mobility.ĮcoRV is reportedly more prone than its isoschizomer Eco32I to delete a base after cleavage.
Sticky ends from different Bpu10I sites may not be compatible.
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